University of Tehran Press Iranian Journal of Biosystem Engineering 2008-4803 50 2 2019 08 23 Potential Use of Some Liquid Natural Media for the Production of Blastospores of Entomopathogenic Fungi Metarhizium anisopliae and Beauveria bassiana Potential Use of Some Liquid Natural Media for the Production of Blastospores of Entomopathogenic Fungi Metarhizium anisopliae and Beauveria bassiana 489 497 72594 10.22059/ijbse.2019.271507.665131 FA Marzieh Rashid Ph.D. student, Department of Plant Protection, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran Reza Talaei-Hassanloui Professor, Department of Plant Protection, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran Faramarz Khodaiyan Professor, Department of Food Science and Technology, Faculty of Agricultural Engineering and Technology, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran Mark Goettel Research Scientist, Lethbridge Research Center, Lethbridge, Alberta, Canada Journal Article 2019 01 16 In the present study, the potential of natural ingredients such as extract of pug, carrot pulp, permeate and fish waste were investigated to produce blastospores of two fungi Beauveria bassiana and Metarhizium anisopliae. SDB and PDB culture media were considered as control. In the case of absence of the initial pH for culture media, the highest production of blastospores was recorded in SDB medium and permeate extract for B. bassiana and M. anisopliae respectively, in three days. In the second case, with the same initial pH (5.6) for culture media, the maximum production of blastospores was observed in the extract of fish waste (3 × 107 blastospore /ml medium) and in the carrot pulp extract (6 × 106 blastospore /ml medium) for B. bassiana and M. anisopliae respectively. In the next step, wheat bran and permeate (4% and 8%) extracts were added to selected medium fish waste and carrot pulp. Addition of extract of permeate (8%) had the greatest effect on increasing blastospor production. In the last step, mixed different salts were added and reduced blastospore production in fungus B. bassiana while increased in M. anisopliae compared to the control medium. Finally, two optimum media were introduced for the production of two entomopathogenic fungi. In the present study, the potential of natural ingredients such as extract of pug, carrot pulp, permeate and fish waste were investigated to produce blastospores of two fungi Beauveria bassiana and Metarhizium anisopliae. SDB and PDB culture media were considered as control. In the case of absence of the initial pH for culture media, the highest production of blastospores was recorded in SDB medium and permeate extract for B. bassiana and M. anisopliae respectively, in three days. In the second case, with the same initial pH (5.6) for culture media, the maximum production of blastospores was observed in the extract of fish waste (3 × 107 blastospore /ml medium) and in the carrot pulp extract (6 × 106 blastospore /ml medium) for B. bassiana and M. anisopliae respectively. In the next step, wheat bran and permeate (4% and 8%) extracts were added to selected medium fish waste and carrot pulp. Addition of extract of permeate (8%) had the greatest effect on increasing blastospor production. In the last step, mixed different salts were added and reduced blastospore production in fungus B. bassiana while increased in M. anisopliae compared to the control medium. Finally, two optimum media were introduced for the production of two entomopathogenic fungi.

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