University of Tehran Press Iranian Journal of Biosystem Engineering 2008-4803 51 1 2020 03 20 Studying of the effect of low density poly ethylene (LDPE) based antimicrobial nanocomposite packaging containing TiO2 nanoparticles on the shelf-life extension of button mushroom (Agaricus bisporus) Studying of the effect of low density poly ethylene (LDPE) based antimicrobial nanocomposite packaging containing TiO2 nanoparticles on the shelf-life extension of button mushroom (Agaricus bisporus) 195 209 73597 10.22059/ijbse.2019.287876.665216 FA Hadi Almasi Associate Professor, Department of Food Science and Technology, Faculty of Agriculture, Urmia University, Urmia, Iran Behbood Pourfathi R&amp;D member of Shirin Asal food industry Co, Tabriz, Iran Roghayeh Mokhtari Zonouzi MSc graduate, Department of Food Science, Faculty of Agriculture, Islamic Azad university of Tabriz, Tabriz, Iran Journal Article 2019 09 02     The aim of this research was to develop an antimicrobial active packaging for shelf life extension of button mushrooms (<em>Agaricus bisporus</em>). TiO₂ nanoparticles at three levels of 0, 0.5 and 1.5% were added to the low density polyethylene (LDPE) film and nanocomposite films were produced by blowing extrusion method. These films were used for packaging of fresh mushrooms. Also, UV irradiation was used on the packaged samples for 15 min to stimulate the antimicrobial activity of the films. Evaluation of antimicrobial properties of films revealed that the inhibitory effect against growth of <em>E. coli</em> and <em>S. aureus</em> increased by increasing TiO₂ content and also by UV irradiation. Mushroom samples were stored for 15 days at refrigerator and physicochemical and microbial tests were applied on them. Weight loss, decreasing of phenolic compounds and also losing of vitamin C were decreased by using nanocomposite films and this effect increased by increasing TiO₂ content. Browning index and color difference with fresh mushroom was the lowest for 1.5% TiO₂ loaded and UV irradiated samples. Microbial counts of mushrooms increased during storage but active films were able to control this increment. Generally, this research indicated that the using of TiO₂ in the LDPE film as a non-contact active packaging along with UV irradiation is able to increase the shelf life of mushroom and preserve its quality attributes during the supply period.      The aim of this research was to develop an antimicrobial active packaging for shelf life extension of button mushrooms (<em>Agaricus bisporus</em>). TiO₂ nanoparticles at three levels of 0, 0.5 and 1.5% were added to the low density polyethylene (LDPE) film and nanocomposite films were produced by blowing extrusion method. These films were used for packaging of fresh mushrooms. Also, UV irradiation was used on the packaged samples for 15 min to stimulate the antimicrobial activity of the films. Evaluation of antimicrobial properties of films revealed that the inhibitory effect against growth of <em>E. coli</em> and <em>S. aureus</em> increased by increasing TiO₂ content and also by UV irradiation. Mushroom samples were stored for 15 days at refrigerator and physicochemical and microbial tests were applied on them. Weight loss, decreasing of phenolic compounds and also losing of vitamin C were decreased by using nanocomposite films and this effect increased by increasing TiO₂ content. Browning index and color difference with fresh mushroom was the lowest for 1.5% TiO₂ loaded and UV irradiated samples. Microbial counts of mushrooms increased during storage but active films were able to control this increment. Generally, this research indicated that the using of TiO₂ in the LDPE film as a non-contact active packaging along with UV irradiation is able to increase the shelf life of mushroom and preserve its quality attributes during the supply period.  https://ijbse.ut.ac.ir/article_73597_986b0ac843b6909522bd6d10b1f2e2d0.pdf