Effect of pre-treating vinasse and inoculum on bio-hydrogen production and production capacity of its co-products in Khuzestan

Document Type : Research Paper

Authors

1 Department of Biosystem engineering Faculty of Agriculture, Shahid Chamran university of Ahvaz

2 Department of Biosystem engineering, Faculty of agriculture, Shahid Chamran university of Ahvaz

3 Department of Agricultural Mechanization and Biosystems Engineering, Shushtar Branch, Islamic Azad University

4 Department of Biology, Faculty of Sciences, Shahid Chamran University of Ahvaz

5 Department of Chemical and Biochemical Engineering, Technical university of Denmark

Abstract

Abstract: Vinasse, the effluent of alcohol distilleries, is a highly polluting waste. The high volume of vinasse and its unpleasant odor, along with its acidic properties and high pollution load, has made it one of the most important environmental challenges in Khuzestan. The use of dark fermentation to treat this wastewater is one of the cheapest and most environmentally friendly methods. Hydrogen is a clean fuel and considered as a carrier of renewable energy, if produced biologically. In the present study, the batch production of bio-hydrogen from vinasse by dark fermentation was studied. The effect of inoculum pretreatment (anaerobic digested sludge) and substrate pretreatment (vinasse) on hydrogen production yield were also investigated. The results showed that the use of chloroform as an inoculum pretreatment had increased hydrogen yield very significantly which lead to a yield of 47.8 ± 3.6 Nml/g of initial volatile solids (VS). Also, sodium hydroxide application had a significant effect on vinasse sugars hydrolysis, and the hydrogen production efficiency was increased up to 100 percent by 99.5 ± 0.1 Nml/ g of initial VS. In all treatments, acetic acid and butyric acid were identified as the dominant organic acids in the final fermentation broth. Finally, according to laboratory results and available field information, the production potential of bio-hydrogen, acetic and butyric acid from. vinasse produced in Khouzestan in 2021 was calculated to be 0.003, 0.153 and 0.132 km3, respectively. The results showed that the production of bio-hydrogen from vinasse by dark fermentation is a very efficient method due to the high potential of this gas production.

Keywords

References

Ministry of Jihad Agriculture. (2019). Agricultural Statistics from 2018 to 2019,Volume One: Crops. p.68.(In Farsi)
Antonopoulou, G., Dimitrellos, G., Beobide, A. S., Vayenas, D., & Lyberatos, G. (2015). Chemical pretreatment of sunflower straw biomass: The effect on chemical composition and structural changes. Journal of Waste and Biomass Valorization, 6(5), 733-746.
Baeyens, J., Zhang, H., Nie, J., Appels, L., Dewil, R., Ansart, R., & Deng, Y. (2020). Reviewing the potential of bio-hydrogen production by fermentation. Journal of Renewable and Sustainable Energy Reviews, 131, 11-23.
Banu, J. R., Kavitha, S., Kannah, R. Y., Bhosale, R. R., & Kumar, G. (2020). Industrial wastewater to biohydrogen: Possibilities towards successful biorefinery route. Journal of Bioresource Technology, 298, 122378.
Bernal, A. P., Dos Santos, I. F. S., Silva, A. P. M., Barros, R. M., & Ribeiro, E. M. (2017). Vinasse biogas for energy generation in Brazil: An assessment of economic feasibility, energy potential and avoided CO2 emissions. Journal of Cleaner Production, 151, 271-260.
Boboescu, I. Z., Gherman, V. D., Mirel, I., Pap, B., Tengölics, R., Rákhely, G., Kovács, K. L., Kondorosi, É., & Maróti, G. (2014). Simultaneous biohydrogen production and wastewater treatment based on the selective enrichment of the fermentation ecosystem. International Iournal of Hydrogen Energy, 39(3), 1502-1510.
Bundhoo, M. Z., Mohee, R., & Hassan, M. A. (2015). Effects of pre-treatment technologies on dark fermentative biohydrogen production: A review. Journal of Environmental Management, 157, 20-48.
Bundhoo, Z. M. (2019). Potential of bio-hydrogen production from dark fermentation of crop residues: A review. International Journal of Hydrogen Energy, 44(32), 17362-17364.
Carrillo-Reyes, J., Buitrón, G., Moreno-Andrade, I., Tapia-Rodríguez, A. C., Palomo-Briones, R., Razo-Flores, E., Aguilar-Juárez, O., Arreola-Vargas, J., Bernet, N., & Braga, A. F. M. (2020). Standardized protocol for determination of biohydrogen potential. Methods X, 7, 100754.
Chaganti, S.R., Lalman, J.A., & Heath, D.D.(2012). 16 S rRNA gene based analysis of the microbial diversity and hydrogen production in three mixed anaerobic cultures. International Journal of Hydrogen Energy, 37(11), 9002-9017.
Chang, S., Li, J. Z., & Liu, F. (2011). Evaluation of different pretreatment methods for preparing hydrogen-producing seed inocula from waste activated sludge. Journal of Renewable Energy, 36(5), 1517-1522.
Da Silva, A. N., Macêdo, W. V., Sakamoto, I. K., Pereyra, D. d. L. A. D., Mendes, C. O., Maintinguer, S. I., Caffaro Filho, R. A., Damianovic, M. H. Z., Varesche, M. B. A., & De Amorim, E. L. C. (2019). Biohydrogen production from dairy industry wastewater in an anaerobic fluidized-bed reactor. Journal of Biomass and Bioenergy, 120, 257-264.
Federation, W. E. & Association, A. (2005). Standard methods for the examination of water and wastewater. American Public Health Association (APHA). Washington, DC, USA.
Food and Agriculture Organization. (2019). FAOSTAT statistical database. Retrived July 6, 2021, from https://www.fao.org/faostat/en/#data/QCL.
Fu, S. F., Xu, X. H., Dai, M., Yuan, X. Z., & Guo, R. B. (2017). Hydrogen and methane production from vinasse using two-stage anaerobic digestion. Journal of Process Safety and Environmental Protection, 107, 81-86.
Gadhe, A., Sonawane, S. S., & Varma, M. N. (2014). Kinetic analysis of biohydrogen production from complex dairy wastewater under optimized condition. International Journal of Hydrogen Energy, 39(3), 13006-13014.
García-Depraect, O., Rene, E. R., Diaz-Cruces, V. F., & León-Becerril, E. (2019). Effect of process parameters on enhanced biohydrogen production from tequila vinasse via the lactate-acetate pathway. Journal of Bioresource Technology, 273, 618-626.
Guo, W. Q., Ren, N. Q., Wang, X. J., Xiang, W. S., Meng, Z. H., Ding, J., Qu, Y. Y., & Zhang, L. S. (2008). Biohydrogen production from ethanol-type fermentation of molasses in an expanded granular sludge bed (EGSB) reactor. International Journal of Hydrogen Energy, 33(19), 4981-4988.
Hoarau, J., Caro, Y., Grondin, I., & Petit, T. (2018). Sugarcane vinasse processing: Toward a status shift from waste to valuable resource. A review. Journal of Water Process Engineering, 24, 11-25.
Hu, B. & Chen, S. (2007). Pretreatment of methanogenic granules for immobilized hydrogen fermentation. International Journal of Hydrogen Energy, 32(15), 3266-3273.
Hwang, M.H., Jang, N.J., Hyun, S.H., & Kim, I.S. (2004). Anaerobic bio-hydrogen production from ethanol fermentation: the role of pH. Journal of Biotechnology, 111(3), 297-309.
Janke, L., Leite, A.F., Batista, K., Silva, W., Nikolausz, M., Nelles, M., & Stinner, W. (2016). Enhancing biogas production from vinasse in sugarcane biorefineries: effects of urea and trace elements supplementation on process performance and stability. Journal of Bioresource Technology, 217, 10-20.
Júnior, A. D. N. F., Etchebehere, C., & Zaiat, M. (2015). Mesophilic hydrogen production in acidogenic packed-bed reactors (APBR) using raw sugarcane vinasse as substrate: Influence of support materials. Journal of Anaerobe, 34, 94-105.
Luo, G., Karakashev, D., Xie, L., Zhou, Q., & Angelidaki, I. (2011). Long‐term effect of inoculum pretreatment on fermentative hydrogen production by repeated batch cultivations: Homoacetogenesis and methanogenesis as competitors to hydrogen production. Journal of Biotechnology and Bioengineering, 108(8), 1816-1827.
Luo, G., Xie, L., Zou, Z., Wang, W., & Zhou, Q. (2010). Evaluation of pretreatment methods on mixed inoculum for both batch and continuous thermophilic biohydrogen production from cassava stillage. Journal of Bioresource Technology, 101 (3), 959-964.
Mishra, P., Thakur, S., Mahapatra, D. M. Ab., Wahid, Z., Liu, H., & Singh, L. (2018). Impacts of nano-metal oxides on hydrogen production in anaerobic digestion of palm oil mill effluent–A novel approach. International Journal of Hydrogen Energy, 43(5), 2666-2676.
Mohammadi, P., Ibrahim, S., Annuar, M. S. M., & Law, S. (2011). Effects of different pretreatment methods on anaerobic mixed microflora for hydrogen production and COD reduction from palm oil mill effluent. Journal of Cleaner Production, 19(14), 1654-1658.
Mohan, S. V. (2009). Harnessing of biohydrogen from wastewater treatment using mixed fermentative consortia: process evaluation towards optimization. International Journal of Hydrogen Energy, 34(17), 7460-7474.
Monlau, F., Barakat, A., Trably, E., Dumas, C., Steyer, J. P., & Carrère, H. (2013). Lignocellulosic materials into biohydrogen and biomethane: impact of structural features and pretreatment. Journal of Critical Reviews in Environmental Science and Technology, 43(3), 260-322.
Nath, K., Muthukumar, M., Kumar, A., & Das, D. (2008). Kinetics of two-stage fermentation process for the production of hydrogen. International Journal of Hydrogen Energy, 33(4), 1195-1203.
Náthia-Neves, G., De Alencar Neves, T., Berni, M., Dragone, G., Mussatto, S. I., & Forster-Carneiro, T. (2018). Start-up phase of a two-stage anaerobic co-digestion process: hydrogen and methane production from food waste and vinasse from ethanol industry. Journal of Biofuel Research, 5(2), 813-820.
Pereira, I. Z., Dos Santos, I. F. S., Barros, R. M., E Silva,  H. L. d. C., Tiago Filho, G. L., & E Silva, A. P. M. (2020). Vinasse biogas energy and economic analysis in the state of São Paulo, Brazil. Journal of Cleaner Production, 260, 118-121.
Rafieenia, R., Lavagnolo, M. C., & Pivato, A. (2018). Pre-treatment technologies for dark fermentative hydrogen production: current advances and future directions. Journal of Waste Management, 71, 734-748.
Razi alcohol company website. (2021). Comodity detail vinass. Retrieved July 6, 2021, from http://www.ya-razi.com.(In Farsi).
Rego, G. C., Ferreira, T. B., Ramos, L. R., De Menezes, C. A., Soares, L. A., Sakamoto, I. K., Varesche, M. B. A., & Silva, E. L. (2020). Bioconversion of pretreated sugarcane vinasse into hydrogen: new perspectives to solve one of the greatest issues of the sugarcane biorefinery. Journal of Biomass Conversion and Biorefinery, 1-15.
Sánchez, F. E., Fuess, L. T., Cavalcante, G. S., Adorno, M. Â. T., & Zaiat, M. (2021). Value-added soluble metabolite production from sugarcane vinasse within the carboxylate platform: An application of the anaerobic biorefinery beyond biogas production. Journal of Fuel, 286 (1), 119378.
Santos, S. C., Rosa, P. R. F., Sakamoto, I. K., Varesche, M. B. A., & Silva, E. L. (2014b). Organic loading rate impact on biohydrogen production and microbial communities at anaerobic fluidized thermophilic bed reactors treating sugarcane stillage. Journal of Bioresource Technology, 159, 55-63.
Silva Neto, J. V. & Gallo, W. L. (2021). Potential impacts of vinasse biogas replacing fossil oil for power generation, natural gas, and increasing sugarcane energy in Brazil. Journal of Renewable and Sustainable Energy Reviews, 135, 110281.
Singh, L. & Wahid, Z. A. (2015). Methods for enhancing bio-hydrogen production from biological process: A review. Journal of Industrial and Engineering Chemistry, 21, 70-80.
Sivagurunathan, P., Kumar, G., Bakonyi, P., Kim, S. H., Kobayashi, T., Xu, K. Q., Lakner, G., Tóth, G., Nemestóthy, N., & Bélafi-Bakó, K. (2016). A critical review on issues and overcoming strategies for the enhancement of dark fermentative hydrogen production in continuous systems. International Journal of Hydrogen Energy, 41(6), 3820-3836.
Soares, J. F., Confortin, T. C., Todero, I., Mayer, F. D., & Mazutti, M. A. (2020). Dark fermentative biohydrogen production from lignocellulosic biomass: Technological challenges and future prospects. Journal of Renewable and Sustainable Energy Reviews, 117, 109484.
Sridevi, K., Sivaraman, E., & Mullai, P. (2014). Back propagation neural network modelling of biodegradation and fermentative biohydrogen production using distillery wastewater in a hybrid upflow anaerobic sludge blanket reactor. Journal of Bioresource Technology, 165, 233-240.
Sydney, E. B., Larroche, C., Novak, A. C., Nouaille, R., Sarma, S. J., Brar, S. K., Letti, L. A. J., Soccol, V. T., & Soccol, C. R. (2014). Economic process to produce biohydrogen and volatile fatty acids by a mixed culture using vinasse from sugarcane ethanol industry as nutrient source. Journal of Bioresource Technology, 159, 380-386.
Tena, M., Luque, B., Perez, M., & Solera, R. (2020). Enhanced hydrogen production from sewage sludge by cofermentation with wine vinasse. International Journal of Hydrogen Energy, 45(32), 15977-15984.
Tena, M., Perez, M., & Solera, R. (2021). Effect of hydraulic retention time on hydrogen production from sewage sludge and wine vinasse in a thermophilic acidogenic CSTR: A promising approach for hydrogen production within the biorefinery concept. International Journal of Hydrogen Energy, 46(11), 7810-7820.
Trchounian, K., Sawers, R. G., & Trchounian, A. (2017). Improving biohydrogen productivity by microbial dark-and photo-fermentations: Novel data and future approaches. Renewable and Sustainable Energy Reviews, 80, 1201-1216.
Urbaniec, K. & Bakker, R. R. (2015). Biomass residues as raw material for dark hydrogen fermentation–A review. International Journal of Hydrogen Energy, 40(9), 3648-3658.
Usman, T. M., Banu, J. R., Gunasekaran, M., & Kumar, G. (2019). Biohydrogen production from industrial wastewater: An overview. Journal of Bioresource Technology Reports, 7, 100287.
Viana, M., Dams, R., Pinheiro, B., Leitão, R., Santaella, S., & Dos Santos, A. (2019). The source of inoculum and the method of methanogenesis inhibition can affect biological hydrogen production from crude glycerol. Journal of Bioenergy Research, 12(3), 742-733.
Wang, J. & Wan, W. (2008). Comparison of different pretreatment methods for enriching hydrogen-producing bacteria from digested sludge. International Journal of Hydrogen Energy, 33(12), 2934-2941.
Zheng, Y., Zhao, J., Xu, F., & Li, Y. (2014). Pretreatment of lignocellulosic biomass for enhanced biogas production. Journal of Progress In Energy and Combustion Science, 42, 35-53.
Iranian Journal of Biosystems Engineering
Volume 53, Issue 1
April 2022
Pages 1-23

Files

History

  • Receive Date: 10 June 2021
  • Revise Date: 09 April 2022
  • Accept Date: 10 April 2022

Share

How to cite

Statistics