Accumulation of Beta-carotene in pure microalgae of Dunaliella Salina and the mixed cultures of Caspian Sea under nitrogen starvation

Document Type : Research Paper

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Abstract

Beta-carotene is a carotenoid and known as the most famous natural pigment. Microalgae are the most important sources for beta-carotene production. Due to the high sterilization cost of pure culture, in this study for the first time, growth, amounts of beta-carotene and chlorophyll in Caspian Sea microalgae under nutrient deficiencies stress, have been measured and compared with pure microalgae of Dunaliella Salina.
The initial and final concentrations of beta-carotene under nitrogen starvation (after 188 hours) was 7.5, 14.8 and 7.0 and 13.5 mol Beta-Carotene/g Protein for pure and mixed culture, respectively. Under nitrogen starvation the final protein concentration of microalgae Dunaliella Salina was increased from 341.8 to 950.1 mg/L. However, for Caspian Sea mixed microalgae the concentration was reached from 357.1 to 1010.0 mg/L. Operating conditions was the same for both samples as follows: pH=7.5, temperature 24-26 ° C and stirring at 160 rpm.
This study demonstrated that the accumulated Beta-carotene in Caspian Sea microalgae was comparable to the obtained values of pure microalgae of Dunaliella Salina. Therefore, mixed microalgae culture can be used for Beta-carotene production which is preferable and more economical than sterile systems and consequently, the possibility of process industrialization will be increased

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References

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Iranian Journal of Biosystems Engineering
Volume 47, Issue 4 - Serial Number 4
January 2017
Pages 725-721

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  • Receive Date: 11 April 2016
  • Revise Date: 04 October 2016
  • Accept Date: 07 November 2016

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