The Effects of Aqueous Extracts of Propolis Obtained from Different Extraction Methods as a Natural Preservative on Raw Milk

Document Type : Research Paper

Authors

1 Department of Food Science, Agricultural Science Faculty, Guilan University. Rasht-Iran

2 Food technologies group, Chemical technologies department, Iranian research organization for science and technologies, Tehran, Iran

Abstract

The effect of aqueous extracts of propolis gained using different extraction methods (soaking, Soxhlet, microwave and ultrasound) on the raw milk quality was investigated. Chemical (pH and acidity), microbial (total count, coliform, yeast and mold) and sensory (color and odor) properties were evaluated at 30 and 5 for 12 and 96 h, respectively. The highest amount of total phenolic compounds (7.4±0.1 (g/100g) and the antioxidant activity (52.0±1.8%) was observed in the Soxhlet extract. Despite the lowest amount of total phenolic compounds and antioxidant activity (2.6±0.6 g /100 g and 26±2.2%, respectively), soaking showed a more appropriate effect regarding growth inhibition of total bacterial counts (6.0±0.0 and 6.9±0.0 log cfu/ml), yeasts (4.5±0.0 and 4.0±0.0 log cfu/ml) and coliforms (5.1±0.1 and 4.1±0.0 log cfu/ml) at both temperatures of 5 and 30 compared to the control group showing the better performance of this methods in the extraction of the effective inhibiting compounds. The propolis extracts obtained from different extraction methods were effective in controlling pH and acidity in both temperatures during storage compared to the control samples. No organoleptic changes were observed during storage of the samples. Propolis extract (except extract gained by Soxhlet) extended the shelf life of milk at 30 and 5 for 3 and 24 h, respectively.

Keywords

References

AL-Ani, I., Zimmermann, S., Rechling, J. & Wink M. (2018). Antimicrobial activities of European propolis collected from various geographic origins alone and in combination with antibiotics. Medicines, 5(1), 2-19.
Briones-Labarca, V., Plaza-Morales, M., Giovagnoli-Vicuna, C. & Jamett, F. (2015). High hydrostatic pressure and ultrasound extractions of antioxidant compounds, sulforaphane and fatty acids from Chilean papaya (Vasconcellea pubescens) seeds: Effects of extraction conditions and methods. LWT-Food Science and Technology, 60(1), 525–534.
Cavalaro, R.I., Cruz, R.G., Dupont, S., Bell, J.M.L.N.M. & Vieira T.M.F.S. (2019). In vitro and in vivo antioxidant properties of bioactive compounds from green propolis obtained by ultrasounds-assisted extraction. Journal of Food Chemistry, 4, 100054- 100062.
Chemat, F., Rombaut, N., Sicaire, A. G., Meullemiestre, A., Fabiano-Tixier, A. S. & Abert- Vian, M. (2017). Ultrasound assisted extraction of food and natural products. Mechanisms, techniques, combinations, protocols and applications. A review. Ultrasonics Sonochemistry, 34, 540–560.
Cunha, I.B.S., Sawaya, A.C.H.F., Caetano, F.M., Shimizu, M.T., Marcucci, M.C., Drezza, F.T., Povia, G.S. & Carvalho, P.O. (2004). Factors that influence the yield and composition of Brazilian propolis extracts. Journal of Brazilian Chemical Society, 15,964-970.
EI-Deeb, A.M. (2017). Utilization of propolis extract as a natural preservative in raw milk. Journal of Food and Dairy Science, 8(8), 315-321.
Gheibi, S., Pourfarzad, A. & Mehregan Nikoo, A. (2020). Extraction of propolis extract and investigation of its effect on quality and shelf life of unpasteurized Doogh. Animal Production Research, 9(3), 99-111. (In Farsi).
Hamzah, N. & Leo, Ch.P. (2015). Microwave-Assisted Extraction of Trigona Propolis: The Effects of Processing Parameters. International Journal of Food Engineering, 11(6), 43-54.
Huang, S., Zhang, C., Wang, K., Li, G.Q. & Hu, F. (2014). Recent advances in the chemical composition of propolis. Journal of Molecules, 19, 19610-19632.
Institute of standards and industrial research of Iran. (2006) Milk and milk products- Determination of titrable acidity and pH value- Test method. National standard No: 2852, 1st.Edition. Retrieved Februrary 13, 2021, from http://isom.isiri.gov.ir/nst.
Institute of standards and industrial research of Iran. (2007). Microbiology of food and animal feeding stuffs – Horizontal method for the enumeration of coliforms – Colony-count technique. National standard No: 9263, 1st.Edition. Retrieved February 13, 2021, from http://isom.isiri.gov.ir/nst.
Institute of standards and industrial research of Iran. (2007). Milk and milk products –Enumeration of colony-forming units of yeasts and/or molds-colony-count technique at 25°C. National standard No: 10154, 1st.Edition. Retrieved February 13, 2021, from http://isom.isiri.gov.ir/nst.
Institute of standards and industrial research of Iran. (2015). Microbiology of food and animal feed- a Comprehensive method for searching for and total count method of the most probable detection. National standard No: 5272, 1st.Edition. Retrieved February 13, 2021, from http://isom.isiri.gov.ir/nst.
Khezri, M., Rostami, S., Riseh, R.S. & Alizadeh, A. (2008). Effect of propolis and clotrimazole on controlling aflatoxin in pistachio. International Journal of Agriculture and Biology, 8(5), 606-608.
Kubiliene, L., Laugaliene, V., Pavilonis, A., Maruska, A., Majiene, D., Barcauskaite, K. & Savickas, A. (2015). Alternative preparation of propolis extracts: Comparison of their composition and biological activities. BMC Complementary and Alternative Medicine, 15(1), 156- 166.
Mirzoeva, O.K., Grishanin, R.N. & Colder, P.C. (1997). Antimicrobial action of propolis and some of its components. Microbiological Research, 152, 239-246.
Pobiega, K., Krasniewska, K., Derewiaka, D. & Gniewosz M. (2019). Comparison of the antimicrobial activity of propolis extracts obtained by means of various extraction methods. Journal of Food Science Technology, 56(12), 5386-5395.
Said, S.A., Khan, S.A., Ahmed, I. & Ali, H.S. (2006). Chemical composition of Egyption and UAE propolis. Pakistan Journal of Pharmaceutical Sciences, 19, 58-61.
Schmidt, K., Stupar, J., Shirley, J., Adapa, S. & Sukup, D. (1996). Factors affecting titratable acidity in raw milk. Dairy Day, 340, 1984-2014.
Sheikhi Koohsar, A. A., Sayyed-Alangi, S. Z., Shamloofar, M. & Sharifian, S. (2018). Effect of different extracts of Iranian propolis on shelf-life of silver crap (Hypophthalmichthys molitrix) fillet in the refrigerator. Journal of Iranian Food science and Technology, 15(3), 51-65.
Singleton, V.L. & Rossi, J.R. (1965). Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid. American Journal of Enology and Viticulture, 16, 144-158.
Trusheva, B., Trunkova, D. & Bankova, V. (2007). Different extraction methods of biologically active components from propolis: a preliminary study. Chemistry Central Journal, 1(13), 1-13.
Woisky R.G. & Salatino A. (1998). Analysis of propolis: some parameters and procedures for chemical quality control. Journal of Apicultural Research, 37, 99-105.
Iranian Journal of Biosystems Engineering
Volume 52, Issue 2
June 2021
Pages 211-221

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History

  • Receive Date: 28 November 2020
  • Revise Date: 16 February 2021
  • Accept Date: 24 February 2021

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