Optimization of dextran production by Leuconostoc mesenteroides in dairy fermented beverage

Document Type : Research Paper

Authors

1 University of Tehran

2 university of tehran

Abstract

Some of lactic acid bacteria are able to produce extracellular polysaccharide through fermentation process. In the food industry, these polysaccharides are frequently used in beverages due to their water binding capacity and increasing viscosity. In addition to technological benefits, certain exopolysaccharides (EPS) have health effect on human, such as cholesterol lowering effect, immune-stimulatory effect and prebiotic activity. In this study, EPS (Dextran) production in dairy fermented beverage by leuconostoc mesenteroides and Synergistic efficacy of lactococcus lactis on dextran production have been investigated. Production of dextran in milk was optimized using response surface methodology with four independent factor, including: fermentation temperature (20-40 °C), fermentation time (15-35 hours), L.mesenteroides-L.lactis inoculation ratio (10:90 - 90:10) and percent of sucrose (0-6%).
According to the findings 20 °C, 20 hours, 4.5% sucrose and inoculation ratio of L.mesenteroides-L.lactis 50:50 was optimum condition for dextran production and the experimental results confirmed the predicted amount of dextran production in optimum condition.

Keywords

Main Subjects


Ai, L., Zhang, H., Guo, B., Chen, W., Wu, Z., & Wu, Y. (2008). Preparation, partial characterization and bioactivity of exopolysaccharides from Lactobacillus casei LC2W. Carbohydrate Polymers74(3), 353-357.‏
Azarikia, F., & Abbasi, S. (2010). On the stabilization mechanism of Doogh (Iranian yoghurt drink) by gum tragacanth. Food Hydrocolloids, 24(4), 358-363.
Bellengier, P., Richard, J., & Foucaud, C. (1997). Associative growth of Lactococcus lactis and Leuconostoc mesenteroides strains in milk. Journal of Dairy Science80(8), 1520-1527.
Boquien, C. Y., Corrieu, G., & Desmazeaud, M. J. (1988). Effect of fermentation conditions on growth of Streptococcus cremoris AM2 and Leuconostoc lactis CNRZ 1091 in pure and mixed cultures. Applied and Environmental Microbiology54(10), 2527-2531.
Duenas, M., Munduate, A., Perea, A., & Irastorza, A. (2003). Exopolysaccharide production by Pediococcus damnosus 2.6 in a semidefined medium under different growth conditions. International Journal of Food Microbiology87(1), 113-120.
Enikeev, R. (2012). Development of a new method for determination of exopolysaccharide quantity in fermented milk products and its application in technology of kefir production. Food Chemistry134(4), 2437-2441.
Hamasaki, Y., Ayaki, M., Fuchu, H., Sugiyama, M., & Morita, H. (2003). Behavior of psychrotrophic lactic acid bacteria isolated from spoiling cooked meat products. Applied and Environmental Microbiology69(6), 3668-3671.
Hassan, A. N., Ipsen, R., Janzen, T., & Qvist, K. B. (2003). Microstructure and rheology of yogurt made with cultures differing only in their ability to produce exopolysaccharides. Journal of Dairy Science86(5), 1632-1638.‏
Hemme, D., & Foucaud-Scheunemann, C. (2004). Leuconostoc, characteristics, use in dairy technology and prospects in functional foods. International Dairy Journal14(6), 467-494.‏
Joudaki, H., Mousavi, M., Safari, M., Razavi, S. H., Emam-Djomeh, Z., & Gharibzahedi, S. M. T. (2013). Scrutinizing the different pectin types on stability of an Iranian traditional drink “Doogh”. International Journal of Biological Macromolecules, 60, 375-382.
Kiani, H., Mousavi, M. E., & Mousavi, Z. E. (2010). Particle stability in dilute fermented dairy drinks: Formation of fluid gel and impact on rheological properties. Food Science and Technology International16(6), 543-551.
Koksoy, A., & Kilic, M. (2004). Use of hydrocolloids in textural stabilization of a yoghurt drink, ayran. Food hydrocolloids, 18(4), 593-600.‏
Mende, S., Peter, M., Bartels, K., Dong, T., Rohm, H., & Jaros, D. (2013). Concentration dependent effects of dextran on the physical properties of acid milk gels. Carbohydrate Polymers98(2), 1389-1396.‏
Mende, S., Rohm, H., & Jaros, D. (2016). Influence of exopolysaccharides on the structure, texture, stability and sensory properties of yoghurt and related products. International Dairy Journal, 52, 57-71.‏
O’sullivan, L., Ross, R. P., & Hill, C. (2002). Potential of bacteriocin-producing lactic acid bacteria for improvements in food safety and quality. Biochimie, 84(5), 593-604.‏
Onilude, A. A., Olaoye, O., Fadahunsi, I. F., Owoseni, A., Garuba, E. O., & Atoyebi, T. (2013). Effects of cultural conditions on dextran production by Leuconostoc sppInternational Food Research Journal20(4).‏
Özer, B. H., & Kirmaci, H. A. (2010). Functional milks and dairy beverages. International Journal of Dairy Technology63(1), 1-15.‏
Pham, P. L., Dupont, I., Roy, D., Lapointe, G., & Cerning, J. (2000). Production of exopolysaccharide by Lactobacillus rhamnosus R and analysis of its enzymatic degradation during prolonged fermentation. Applied and Environmental Microbiology66(6), 2302-2310.‏
Purama, R. K., & Goyal, A. (2008). Identification, effective purification and functional characterization of dextransucrase from Leuconostoc mesenteroides NRRL B-640. Bioresource Technology99(9), 3635-3642.‏
Ruas-Madiedo, P., Hugenholtz, J., & Zoon, P. (2002). An overview of the functionality of exopolysaccharides produced by lactic acid bacteria. International Dairy Journal12(2), 163-171.‏
Samaržija, D., Antunac, N., & Havranek, J. L. (2001). Taxonomy, physiology and growth of Lactococcus lactis: a review. Mljekarstvo51(1), 35-48.‏
Shao, L. I., Wu, Z., Zhang, H., Chen, W., Ai, L., & Guo, B. (2014). Partial characterization and immunostimulatory activity of exopolysaccharides from Lactobacillus rhamnosus KF5. Carbohydrate Polymers107, 51-56.‏
Stiles, M. E. (1994). Bacteriocins produced by Leuconostoc species. Journal of Dairy Science, 77(9), 2718-2724.‏
Teimouri, S., Abbasi, S. and Scanlon, M. G. (2017), Stabilisation mechanism of various inulins and hydrocolloids: Milk–sour cherry juice mixture. Int J Dairy Technol. doi:10.1111/1471-0307.12376.
Welman, A. D. (2014). Exopolysaccharides from fermented dairy products and health promotion. Advances in Fermented Foods and Beverages: Improving Quality, Technologies and Health Benefits, 23.
Yilmaz, M. T., Dertli, E., Toker, O. S., Tatlisu, N. B., Sagdic, O., & Arici, M. (2015). Effect of in situ exopolysaccharide production on physicochemical, rheological, sensory, and microstructural properties of the yogurt drink ayran: An optimization study based on fermentation kinetics. Journal of Dairy Science, 98(3), 1604-1624.
Majumder, A., Bhandari, S., Purama, R. K., Patel, S., & Goyal, A. (2009). Enhanced production of a novel dextran fromLeuconostoc mesenteroides NRRL B-640 by Response Surface Methodology. Annals of Microbiology59(2), 309-315.
Volume 48, Issue 4
December 2017
Pages 399-406
  • Receive Date: 14 August 2016
  • Revise Date: 25 July 2017
  • Accept Date: 29 July 2017
  • First Publish Date: 22 December 2017