Investigation of Properties of Bioactive Peptides Derived from Enzymatic Hydrolysis of Chicken Slaughter Waste

Document Type : Research Paper


1 Graduated MSc of Food Science and Technology, Islamic Azad University, Science and Research Branch,Tehran, Iran.

2 Associate Professor of Food Science and Technology, Islamic Azad University, Science and Research Branch,Tehran, Iran.

3 Assistant Professor of Department of Food Science and Technology, Faculty of Industrial and Mechanical Engineering, Qazvin Branch, Islamic Azad University, Qazvin, Iran


The purpose of this study was to investigate the possibility of producing bioactive peptides from chicken waste proteins by enzymatic hydrolysis. Protein extract of chicken waste was hydrolyzed by Actinidin Cyanocele extract. Then it was estimated the degree of hydrolysis, mean length of the peptide chain and the antioxidant property of the hydrolyzed product. Assay of antioxidant property of protein hydrolyzates showed that in the first four hours, antioxidant property of experiment samples increased to 85%, while with increasing the hydrolysis until 8 hours, the antioxidant activity decreased to 32%. The reason for this reduction can be more hydrolysis and degradation in regions of bioactive peptides that have antioxidant properties. So, the results of this study indicated that enzymatic hydrolysis of chicken waste is a way for production of natural antioxidant compounds that can be considered as drug or additive in food and pharmaceutical industries.


Main Subjects

Boland,  M.  (2013). Kiwifruit proteins and enzymes: actinidin and other significant proteins. Advances in food and nutrition research. 68, 59-80.
Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram of protein-dye binding.         Analytical biochemistry. 72, 248-254.
Englund, P.T., King, T.P., Craig, L.C. & Walti, A.N.D.A. (1968). Ficin. I. Its isolation and characterization. Biochemistry, 7(1), 163-175.
Esmaielpoor, M. ( 2015). Seperation and survey of bioactive properties of peptides from enzymatic hydrolysis of goat milk. Ph.D. dissertation, University ofTehran Science and Research, Food science and technology. (In Farsi).
Ha, M., Bekhit,  A.E.D.A., Carne, A. & Hopkins, D.L.  (2012). Characterisation of commercial papain,
bromelain,  actinidin and zingibain protease preparations and their activities toward meat proteins. Food chemistry, 134(1), 95-105.
Ha,  M., Bekhit,  A.E.D., Carne, A. & Hopkins, D.L. (2013). Characterisation of kiwifruit and asparagus enzyme extracts,  and their activities toward meat proteins.  Food  chemistry, 136(2), 989-998.
Jamdar, S.N., Rajalakshmi, V. & Sharma, A. (2012). Antioxidant and ace inhibitory properties of poultry viscera protein hydrolysate and its peptide fractions.Journal of Food Biochemistry, 36(4), 494-501.
Khatib, N. ( 2010).  Measurment and survey of bioactive properties of peptides from enzymatic treatment camel meat. Master of Science Thesis, University of Esfehan, Agriculture College. (In Farsi).
Kim , E.K., Lee, S.J., Jeon, B.T., Moon, S.H., Kim, B., Park, T.K., Han, J.S. & Park, P.J. (2009).  Purification and characterisation of antioxidative peptides from  enzymatic hydrolysates of venison protein. Food Chemistry,  114(4), 1365-1370.
Korhonen, H. & Pihlanto, A. (2006). Bioactive peptides: production and functionality. International Dairy Journal, 16(5), 945-960.
Lasekan , A.,  Bakar, F.A. & Hashim, D. (2013). Potential of chicken by-products as sources of useful biological resources . Waste  Management, 33(3), 552-565.
Mirzaie, M. (2015). Production and seperation bioactive peptides from enzymatic hydrolysis of sacharomyses serviziye yeast proteins with antioxidant and antimicrobial properties. Ph.D.dissertation,University ofTehran Science and Research, Food science and technology. (In Farsi).
Moller, N. P ., Scholz-Ahrens, K.E., Roos, N. & Schrezenmeir, J. (2008). Bioactive peptides and proteins from foods: indication for health effects. European  Journal of Nutrition, 47(4), 171-182.
Mostafaie, A. & Chelbi, M. ( 2006). Kiwifruit actinidin: purification and survey its amount in native varieties. Journal of Agriculture and Natural Sources Sciences and Techniques, 10(3), 223-230. (In Farsi).
Nikolaev, I.V., Sforza, S., Lambertini, F., Ismailova, D.Y., Khotchenkov, V.P., Volik, V.G., Dossena, A., Popov, V.O. & Koroleva, O.V. (2016). Biocatalytic conversion of poultry processing leftovers: Optimization of hydrolytic conditions and peptide hydrolysate characterization. Food chemistry.197, 611-621.
Ohba, R., Deguchi, T., Kishikawa, M., Arsyad, F., Morimura, S. & Kida, K. (2003). Physiological functions of enzymatic hydrolysates of collagen or keratin contained in livestock and fish waste. Food Science and Technology Research, 9(1), 91-93.
Saiga, A.I., Tanabe, S. & Nishimura, T. (2003). Antioxidant activity of peptides obtained from porcine myofibrillar proteins by protease treatment. Journal of Agricultural and Food chemistry, 51(12), 3661-3667.
Saiga, A., Okumura, T., Makihara , T., Katsuta, S., Shimizu, T., Yamada, R. & Nishimura, T. (2003).  Angiotensin  I-converting enzyme inhibitory peptides in a hydrolyzed chicken breast muscle extract. Journal of Agricultural and Food Chemistry , 51(6), 1741-1745.
Soladoye, O.P., Saldo, J ., Peiro, L., Rovira, A. & Mor-Mur, M. (2015). Antioxidant and angiotensin 1 converting enzyme inhibitory functions from chicken collagen  hydrolysates. Journal of Nutrition & Food Sciences ,5(3), 1-9.
Sugiyama, S., Hirota, A., Okada, C., Yorita, T., Sato, K. & Ohtsuki, K. (2005). Effect of kiwifruit juice on beef collagen. Journal of nutritional science and vitaminology, 51(1), 27-33.