Potential Use of Some Liquid Natural Media for the Production of Blastospores of Entomopathogenic Fungi Metarhizium anisopliae and Beauveria bassiana

Document Type : Research Paper

Authors

1 Ph.D. student, Department of Plant Protection, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

2 Professor, Department of Plant Protection, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

3 Professor, Department of Food Science and Technology, Faculty of Agricultural Engineering and Technology, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

4 Research Scientist, Lethbridge Research Center, Lethbridge, Alberta, Canada

Abstract

In the present study, the potential of natural ingredients such as extract of pug, carrot pulp, permeate and fish waste were investigated to produce blastospores of two fungi Beauveria bassiana and Metarhizium anisopliae. SDB and PDB culture media were considered as control. In the case of absence of the initial pH for culture media, the highest production of blastospores was recorded in SDB medium and permeate extract for B. bassiana and M. anisopliae respectively, in three days. In the second case, with the same initial pH (5.6) for culture media, the maximum production of blastospores was observed in the extract of fish waste (3 × 107 blastospore /ml medium) and in the carrot pulp extract (6 × 106 blastospore /ml medium) for B. bassiana and M. anisopliae respectively. In the next step, wheat bran and permeate (4% and 8%) extracts were added to selected medium fish waste and carrot pulp. Addition of extract of permeate (8%) had the greatest effect on increasing blastospor production. In the last step, mixed different salts were added and reduced blastospore production in fungus B. bassiana while increased in M. anisopliae compared to the control medium. Finally, two optimum media were introduced for the production of two entomopathogenic fungi.

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References

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Iranian Journal of Biosystems Engineering
Volume 50, Issue 2
September 2019
Pages 489-497

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History

  • Receive Date: 16 January 2019
  • Revise Date: 10 February 2019
  • Accept Date: 23 February 2019

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