Evaluation of Antioxidant Activity of Hydroalcoholic Extracts from Pumpkin (Cucurbita moschata D.) Seed, Flesh and Skin

Document Type : Research Paper


1 Sari Agricultural Sciences and Natural Resources University

2 Associated Professor, Department of Biotechnology and Plant Breeding, Sari Agricultural Sciences and Natural Resources University Sari, Iran.

3 Associated Professor, Department of Food Science and Industry, Sari Agricultural Sciences and Natural Resources University Sari, Iran.


The aim of this study is to determine the total phenol and flavonoid content and antioxidant activity of hydroalcoholic extracts from different parts of pumpkin (skin, seed and flesh). The maximum extract production was obtained for flesh (27.83 g extract/100 g powder) while the lowest yield obtained from the seed extract (6.30 g extract/100 g powder). For seed extract, the highest amount of total phenol (25.80 ± 0.69 mg (GAE)/g extract) and flavonoid content (7.24 ± 0.28 mg(QE)/g extract) obtained respectively through the assay of Folin Ciocalteu and aluminum calorimetric and the best antioxidant activity was gained through antioxidant  assays of DPPH (IC50=794.32µg/ml) and β-carotene bleaching (at a dose of 2000 µg/ml it was equivalent to synthetic antioxidant BHA in the concentration of 200 µg/ml). Based on the results of oxidative stability index (OSI) for extracts, the skin extract showed the highest stability (8.58 ± 0.55 h) and no significant difference was observed between the seed and fruit extracts (7.13 ± 0.168 h and 7.33 ± 0.08 h, respectively). The results suggested that hydroalcoholic extracts of various parts of the pumpkin’s fruit, especially its seeds, may serve as a potential source of natural antioxidant for food and pharmaceutical products.


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