Investigation of environmental factors affecting the production of Lipid, Protein, and Betacarotene in Dunaliella salina microalgae

Document Type : Research Paper


1 Department of Biosystem Mechanics, Faculty of Agriculture, Islamic Azad University, Arak Branch, Arak, Iran.

2 Department of Biosystem Mechanics, Faculty of Agriculture, Islamic Azad University, Arak Branch, Arak

3 Department of Biology, Faculty of Science, Farhangian University, Tehran, Iran


Microalgae can produce various products such as biofuel, proteins, and beta-carotene. Knowing the optimal conditions to produce these products is very important. In this research, the effect of environmental parameters on the production rate of each product in Dunaliella salina microalgae was investigated. saltiness (1, 2, and 3 M), pH above and below 7, nitrate concentrations of 0.25 and 0.5 g/L, light intensities of 2500 and 5000 lux, and temperature levels of 25 and 30 °C were studied. According to the results of this study, the highest cell density of algae was at 3 M salinity and pH higher than 7, nitrate concentration equal to 0.25 g/L, light intensity 5000 lux, and temperature 25°C. The same conditions of salinity 3 M, pH of more than 7, nitrate concentration of 0.25 M, light intensity of 5000 lux, and temperature of 25°C prevailed for the maximum production of microalgae biomass. Under these conditions, the highest amount of protein was produced in Dunaliella salina microalgae. But for lipid production, the optimal conditions were at a salinity of 2 M, pH more than 7, nitrate concentration of 0.25 g/L, light intensity of 5000 lux, and temperature of 25°C. Optimum production of betacarotene also occurred at 3 M salinity, pH more than 7, nitrate concentration 0.25 g/L, a light intensity of 5000 lux, and temperature of 30°C. By knowing these conditions, it is possible to achieve the maximum amount of production of products such as biofuel, protein, and betacarotene from Dunaliella salina microalgae.


Main Subjects

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