تأثیر زمان تیمار آنزیمی‌ترانس‌گلوتامیناز میکروبی بر خصوصیات فیلم خوراکی بر پایه ایزوله پروتئین آب‌پنیر

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد، گروه صنایع غذایی، دانشکده علوم دامی و صنایع غذایی، دانشگاه کشاورزی و منابع طبیعی رامین خوزستان

2 عضو هیات علمی گروه علوم و صنایع غذایی، دانشکده علوم دامی و صنایع غذایی، دانشگاه کشاورزی و منابع طبیعی رامین خوزستان

چکیده

در این مطالعه، تأثیر زمان تیمار آنزیمی ترانس­گلوتامیناز میکروبی (MTGase) بر خصوصیات فیلم خوراکی بر پایه ایزوله پروتئین آب­پنیر بررسی شد. نتایج نشان داد که خصوصیات فیلم تیمارشده با آنزیم، متأثر از زمان تیمار آنزیمی محلول تشکیل­دهنده فیلم است. تیمار با زمان کم (1 ساعت) به طور معنی­داری باعث افزایش مقاومت به کشش (TS) و کاهش درصد افزایش طول تا نقطه شکست (EB) در فیلم در مقایسه با نمونه شاهد شد. در فیلم­های تیمارشده، میزان  TS بتدریج با افزایش زمان از 1 به 3 ساعت، کاهش و میزان EB بتدریج افزایش یافت. نفوذپذیری به بخار آب فیلم شاهد در مقایسه با فیلم تیمار شده با آنزیم به مدت 1 ساعت از gm-1s-1pa-110-10×6/3 به gm-1s-1pa-110-10×31/2 و حلالیت در آب آن از 07/37 به 97/19 درصد کاهش یافت؛ اما همزمان با افزایش زمان، حلالیت در آب و نفوذپذیری به بخار آب افزایش یافت.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The influence of enzymatic treatment time of microbial transglutaminase on the properties of edible film based on whey protein isolate

نویسندگان [English]

  • Farzaneh Kooravand 1
  • Hosein Jooiandeh 2
  • Hasan Barzgar 2
  • Mohammad Hojjati 2
چکیده [English]

The effect of enzymatic treatment time of microbial transglutaminase (MTGase) on the characteristics of whey protein isolate (WPI) films was investigated. Results shown that the properties of MTGase-treated films were affected by increasing the enzymatic treatment time. Enzymatic treatment at lower incubation time, i.e. for 1 h, significantly resulted in higher values of tensile strength (TS) and lower values of elongation at break (EB) as compared to control film. In the MTGase-treated films, the TS values decreased gradually and EB values increased progressively with increasing incubation time from 1 to 3 h. Water vapor permeability (WVP)and total soluble matter(TSM) of control film in comparison with MTGase-treated films decreased from3.6 ×10 10 to 2.31 ×10 10 g m-1 s-1 pa-1 and from 37.07 to 19.97 percent, respectively. However, as the time of enzymatic treatment was increased the TSM and WVP were gradually increased.

کلیدواژه‌ها [English]

  • Edible film
  • Mechanical properties
  • Microbial transglutaminase
  • Whey protein isolate

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مهندسی بیوسیستم ایران
دوره 47، شماره 3
آبان 1395
صفحه 519-527

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  • تاریخ دریافت: 13 خرداد 1395
  • تاریخ بازنگری: 20 خرداد 1395
  • تاریخ پذیرش: 25 خرداد 1395

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