بررسی پتانسیل استفاده از چند محیط کشت‌ طبیعی مایع برای تولید بلاستوسپور‌‌‌‌های دو قارچ بیمارگر حشراتMetarhizium anisopliae و Beauveria bassiana

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشجوی دکترای تخصصی، گروه گیاهپزشکی، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج، ایران

2 استاد، گروه گیاهپزشکی، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج، ایران

3 استاد، گروه صنایع غذایی، دانشکده مهندسی و فناوری کشاورزی، پردیس کشاورزی و منابع طبیعی، دانشگاه تهران، کرج، ایران

4 استاد پژوهش، مرکز تحقیقات لتبریج، لتبریج، آلبرتا، کانادا

چکیده

 در پژوهش حاضر، پتانسیل عملکرد مواد طبیعی شامل عصاره‌های تفاله سیب، تفاله هویج، آب پنیر و ضایعات ماهی برای تولید بلاستوسپورهای دو قارچ Beauveria bassianaوMetarhizium anisopliae  مورد بررسی قرار گرفت. محیط کشت‌های SDB وPDB  به عنوان تیمار مقایسه ای در نظر گرفته شد. در حالت یکسان نبودن pH اولیه محیط کشت‌ها، بیشترین تولید بلاستوسپور برای قارچB. bassiana در محیط SDB  و برای قارچM. anisopliae  در محیط عصاره آب پنیر در مدت سه روز مشاهده گردید. در حالت دوم با pH اولیه یکسان (۶/۵) برای محیط کشت‌ها، بیشترین تولید بلاستوسپور برای قارچ B. bassiana درمحیط عصاره ضایعات ماهی (۱۰۷×۳ بلاستوسپور بر میلی‌لیتر محیط) و برایM. anisopliae  در محیط عصاره تفاله هویج (۱۰۶×۶ بلاستوسپور بر میلی‌لیتر محیط) بود. در گام بعدی، عصاره‌های سبوس گندم و آب پنیر( ۴% و ۸%) به محیط کشت‌های منتخب ضایعات ماهی و تفاله هویج اضافه شد. افزودن عصاره آب پنیر ۸% بیشترین تأثیر در افزایش تولید بلاستوسپور را داشت. در گام آخر، مخلوط نمک‌های مختلف اضافه شد و سبب کاهش تولید بلاستوسپور در قارچB. bassiana و افزایش بلاستوسپور درM. anisopliae  در مقایسه با محیط کشت شاهد گردید. در نهایت دو محیط کشت بهینه برای تولید دو قارچ بیمارگر حشرات معرفی شد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Potential Use of Some Liquid Natural Media for the Production of Blastospores of Entomopathogenic Fungi Metarhizium anisopliae and Beauveria bassiana

نویسندگان [English]

  • Marzieh Rashid 1
  • Reza Talaei-Hassanloui 2
  • Faramarz Khodaiyan 3
  • Mark Goettel 4
1 Ph.D. student, Department of Plant Protection, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
2 Professor, Department of Plant Protection, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
3 Professor, Department of Food Science and Technology, Faculty of Agricultural Engineering and Technology, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
4 Research Scientist, Lethbridge Research Center, Lethbridge, Alberta, Canada
چکیده [English]

In the present study, the potential of natural ingredients such as extract of pug, carrot pulp, permeate and fish waste were investigated to produce blastospores of two fungi Beauveria bassiana and Metarhizium anisopliae. SDB and PDB culture media were considered as control. In the case of absence of the initial pH for culture media, the highest production of blastospores was recorded in SDB medium and permeate extract for B. bassiana and M. anisopliae respectively, in three days. In the second case, with the same initial pH (5.6) for culture media, the maximum production of blastospores was observed in the extract of fish waste (3 × 107 blastospore /ml medium) and in the carrot pulp extract (6 × 106 blastospore /ml medium) for B. bassiana and M. anisopliae respectively. In the next step, wheat bran and permeate (4% and 8%) extracts were added to selected medium fish waste and carrot pulp. Addition of extract of permeate (8%) had the greatest effect on increasing blastospor production. In the last step, mixed different salts were added and reduced blastospore production in fungus B. bassiana while increased in M. anisopliae compared to the control medium. Finally, two optimum media were introduced for the production of two entomopathogenic fungi.

کلیدواژه‌ها [English]

  • fish waste
  • carrot pulp
  • apple pug
  • permeate
  • yield

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