هیدرولیز اسیدی ضایعات نان برای تولید بیواتانول توسط مخمر ساکارومایسس سرویزیه (Saccharomyces cerevisiae)

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه مهندسی فنی کشاورزی. پردیس ابوریحان، دانشگاه تهران. تهران. ایران.

2 گروه مهندسی فنی کشاورزی، پردیس ابوریحان، دانشگاه تهران. تهران. ایران

3 گروه مهندسی فناوری صنایع غذایی، پردیس ابوریحان، دانشگاه تهران، تهران، ایران

4 گروه مهندسی بیوسیستم، دانشکده کشاورزی، دانشگاه تربیت مدرس، تهران، ایران

چکیده

در حالی­که به میزان زیادی ضایعات نان هر ساله تولید می­شود، به دلیل آلودگی به افلاتوکسین، این ضایعات مناسب مصارف غذایی و خوراک دام نیستند. در این پژوهش تولید بیواتانول از ضایعات نان توسط مخمر ساکارومایسس سرویزیه[1] به روش هیدرولیز اسیدی مورد تحقیق قرار گرفت. هیدرولیز اسیدی در دستگاه اتوکلاو انجام شد. اثر غلظت محلول اسیدی و زمان بر میزان گلوکز بررسی گردید. آزمایش­ها در بارگذاری 160 گرم بر لیتر انجام شد. کاهش آلودگی به آفلاتوکسین برای نمونه با بیش­ترین بازده در هیدرولیز اسیدی مورد سنجش قرارگرفت. محلول حاصل از هیدرولیز با استفاده از مخمر ساکارومایسس سرویزیه برای تولید بیواتانول استفاده­گردید. نتایج نشان داد که اثر متغیرهای غلظت اسید و زمان بر میزان گلوکز در سطح 1% معنی­دار است. هیدرولیز در غلظت محلول اسیدی 1 درصد و زمان 20 دقیقه با گلوکز تولیدی 64/80 گرم بر لیتر بیش­ترین مقدار کربوهیدرات را آزاد می­کند. همچنین هیدرولیز اسیدی به ترتیب باعث کاهش100 درصد و 70/20 درصدی آفلاتوکسین B1 و B2شده است. بیشترین درصد تولید بیواتانول در فاز تخمیر نمونه­های هیدرولیز، مقدار 7/4 (%v/v) بود.



[1]. Saccharomyces cerevisiae

کلیدواژه‌ها


عنوان مقاله [English]

Acidic Hydrolysis of Bread Waste for Bio-ethanol Production by Saccharomyces Cerevisiae

نویسندگان [English]

  • Samane Torabi 1
  • Seyed Reza Hassan-Beygi 2
  • Behzad Sattari 3
  • Barat Ghobadian 4
1 Dept. Agro-technology, College of Abouraihan, University of Tehran, Tehran, Iran.
2 ٍِDept. Agro-technology, College of Abouraihan, University of Tehran, Tehran, Iran
3 Dept. Food Industry, College of Aburaihan, University of Tehran, Tehran, Iran
4 Dept. of Bio-system Engineering, College of Agriculture, Tarbiat Modares University, Tehran, Iran
چکیده [English]

In-spite of large amounts production of bread waste annually, this waste is not suitable for food and animal feed applications due to aflatoxin contamination. In this study, bio-ethanol production from bread waste by Saccharomyces cerevisiae was investigated using acidic hydrolysis method. The acidic hydrolysis was performed by an autoclave apparatus. The effect of acidic solution concentration and time on amount of glucose was investigated. The experiments were carried out at loading of 160 g/l. Reduction of aflatoxin was measured for the sample with the greatest yield in acid hydrolysis. The sample obtained from hydrolysis was used for the production of bio-ethanol using Saccharomyces cerevisiae. The results showed that the effect of concentration of acid and time was significant at 1% level on the amount of glucose. Hydrolysis liberated the greatest amount of carbohydrate (80.64 g/l glucose) at the acidic solution concentration of 1% and time of 20 minutes. Also, acidic hydrolysis reduced aflatoxin B1 and B2 by 100% and 20.70%, respectively. The greatest percentage of bio-ethanol production in the fermentation phase of hydrolysis samples was 4.7 (v / v%).

کلیدواژه‌ها [English]

  • Aflatoxin
  • Bio-fuel
  • Bio-Fermentation
  • glucose
Anonymous, 2017. Glucose assay kit. Ziestchem Diagnostics, Tehran, Iran. (In Farsi)
Acanski, M. Pastor, K. Razmovski, R. Vucurovic, V. Psodorov, D. (2014). Bioethanol production from waste bread samples made from mixtures of wheat and buckwheat flours. Journal on Processing and Energy in Agriculture, 18(1), 40-43.
Abedi, M. (2012). Bioethanol production from potato waste. Master of Science Thesis. University of Tehran. Tehran, Iran. (In Farsi).
Caputi, A. Ueda, M. Brown, Th. (1968). Spectrophotometric determination of ethanol in wine. American Journal of Enology and Viticulture, 19, 160-165.
Chambers, PJ. Pretorius, IS. (2010). Fermenting knowledge: the history of winemaking, science and yeast research. EMBO Reports. 11(12), 914-920.
Chiaramonti, D. (2007). Bioethanol: role and production technologies. In P. Ranalli (Ed). Improvement of crop plants for industrial end uses. Springer.(pp. 209-251).
Duku, M. H., GU, S., Hagan, E.B. (2011). A comprehensive review of biomass resources and biofuels potential in Ghana, Renewable and Sustainable Energy Reviews, 14 (1), 515– 514.
Ebrahimi, B. Rahmani, M. (2012). The study of technology development issues obtained by the production and use of biofuels compared to the fossil ones. Journal of development of industrial technology, 10 (19), 27:37. (In Farsi)
Ghorbani, F. Amini, M. Daneshi, A. (2009). Production of ethanol from waste molasses of sugar factories in a discontinuous fermentation system. Journal of Science and technology Environmental, 11 (4). (In Farsi)
Hashem, M. Asseri, T.Y.A. Alamri, S.A. Alrumman, S.A. (2018). Feasibility and Sustainability of Bioethanol Production from Starchy restaurants’ Bio-wastes by New Yeast Strains, Waste and Biomass Valorization .10(4), 1617–1626
Hassan-Beygi, S. R. Istan, V, Ghobadian, B. Aboonajmi, M. (2013). An experimental investigation of Perkins A63544 diesel engine performance using D-Series fuel. Energy Conversion and Management, 76, 356–361.
ISIRI, 6872, 2011. Food and feed stuffs - determination of aflatoxins B&G by HPLC method using immunoaffinity column clean up-Test method. Institute of Standards and Industrial Research of Iran, Tehran, Iran (In Farsi)
Kapdan, I. Kargi, F. Oztekin, R. Argun, H. (2009). Bio-hydrogen production from acid hydrolyzed wheat starch by photo-fermentation using different Rhodobacter sp. International journal of hydroenergy, 34 (5), 2201- 2207.
Khoshpey, B. Farhud, D.D. and Zaini, F. (2011). Aflatoxins in Iran: Nature, hazards and carcinogeicity. Iranian Journal of Public Health, 40 (4), 1-30.
Kim, Y. Jang, J. Park, S. Um, B. (2018). Dilute sulfuric acid fractionation of Korean food waste for ethanol
and lactic acid production by yeast. Waste Management journal, 74 .231–240.
Lee, J. Her, J.Y. Lee, K.G. (2015). Reduction of aflatoxins (B1, B2, G1, and G2) in soybean-based model systems. Food Chemistry  Journal, 189 (15), 45–51.
Licths, F. (2001). Ethanol knowledge: Ethanol Applition. Available at: http:// www.iecbp.com.
Mahmoodi, P. Karimi, K. Taherzade, M. (2018). Efficient conversion of municipal solid waste to biofuel by simultaneous dilute-acid hydrolysis of starch and pretreatment of lignocelluloses. Energy Conversion and Management, 166 (15), 569-578.
Masumiyan, Z. Yavarmanesh, M. Shahidi Noghabi, M. Sadeghi, M. Sohrabi Balsini, M. (2015).  The efficiency of zeolite and citric acid in the control of mold growth and production of Aflaoxin in dry breads wastage across the Mashhad and it's modeling with artificial neural networks method. Science and Food industry of Iran. 12 (48), 99-114. (In Farsi)
Melikoglu, M. Webb, C. (2013). Use of waste bread to produce fermentation products. In: M.R. Kosseva and C. Webb (Eds). Food industry wastes: assessment and recuperation of commodities. Amsterdam: Elsevier Science Publishers,  63–76.
Mena, C. Adenso-Diaz, B. Yurt, O. (2011). The causes of food waste in the supplier–retailer interface. evidences from the UK and Spain. Resources, Conservation and Recycling, 55 (6), 648-658.
Najafi, G. Ghobadian, B. Tavakoli, T. Yusaf, T. (2009). Potential of bioethanol production from agricultural wastes in Iran. Renewable and Sustainable Energy reviews, 13, 1418–1427.
Obeidavi, Z. (2014). Investigation the bioethanol production process and microorganisms' the role in this process. International Conference on Environmental Science, Engineering and Technologies, Tehran, Iran. (In Farsi) From https://www.civilica.com/Paper-CESET01-CESET01_358.html
Pietrzak, W. Kawa-Riejilska, J. (2014). Ethanol fermentation of waste bread using granular starch hydrolyzing enzyme: effect of raw material pretreatment. Fuel, 134 (15), 250-256.
Pietrzak, W. Kawa-Riejilska, J. (2015). Simultaneous scarification and ethanol fermentation of waste wheat–rye bread at very high solids loading: Effect of enzymatic liquefaction conditions. Fuel, 147 (1), 236-242.
Rastegar, H. Shoeibi, Sh. Yazdan-panah, H. AmirAhmadi, M. Mousavi Khaneghah, A. Bovo Campagnollo, F. S. Sant’Ana, A. (2017). Removal of aflatoxin B1 by roasting with lemon juice and/or citric acid in contaminated pistachio nuts. Food Control, 71 ,279-284.
Sattari, B & Karimi, K. (2018). Mucoralean fungi for sustainable production of bioethanol and biologically active molecules. Applied Microbiology and Biotechnology. 102: 1097–1117.
Shahnoushi, N. Firoozzare, A. Jalerajabi, M. Daneshvar, M. Dehghanian, S. (2011). The use of the order logit model in an investigation of the effective factors on bread waste.  Journal of Economic Research. 46 (3), 111-132. 0039-8969. (In Farsi) From https://jte.ut.ac.ir/article_23966.html
Tyner, WE. (2013). Biofuels and food prices: separating wheat from chaff. Global food security, 2 (2), 126-130.
Valentine, J. Clifton-Brown, J. Hastings, A. Robson, P. Allison, G. Smith, P. (2012).  Food vs. fuel: the use of land for lignocellulosic ‘next generation’ energy crops that minimize competition with primary food production. GCB Bioenergy,4 (1),1–19.