استخراج پلی ساکارید پکتین از چوب مرکزی گردو و بررسى بازده و خصوصیات فیزیکوشیمیایى آن

نوع مقاله : مقاله پژوهشی

نویسندگان

استادیار گروه علوم و صنایع غذایی، واحد رودهن، دانشگاه آزاد اسلامی، رودهن، ایران

چکیده

گردو از مهمترین و ارزشمندترین محصولات کشاورزی کشور است. هدف از این پژوهش بررسی تاثیر سه متغیر pH (1، 5/1 و 2)، دما (60، 70 و 80 درجه سلسیوس) و زمان استخراج (60، 90 و 120 دقیقه) بر درصد بازده، درجه استریفیکاسیون و میزان گالاکتورونیک اسید پکتین استخراج شده از ضایعات چوب مرکزی گردوبود که از روش آماری سطح پاسخ استفاده گردید. مطابق با نتایج، شرایط بهینه استخراج با بیشترین بازده استخراج (32/14 درصد)، درجه استریفیکاسیون (03/64 درصد) و گالاکتورونیک اسید (62/63 درصد) متناسب با pH 68/1، دمای 80 درجه سلسیوس و زمان 120 دقیقه بود. روی نمونه­ی بهینه پکتین آزمون­های خاکستر کل، وزن مولکولی، خصوصیات امولسیفایری، رئولوژیکی و طیف سنجی مادون قرمزانجام گرفت. نتایج نشان داد خاکسترکل 14/1 درصد و وزن مولکولی نمونه بهینه پکتین 61/35 کیلو دالتون بود. این نمونه با خواص امولسیفایری مطلوب، رفتار سودوپلاستیک از خود نشان داد و نمودار طیف سنج مادون قرمز آن غنی از حضور گالاکتورونیک اسید بود.

کلیدواژه‌ها


عنوان مقاله [English]

Extraction of Pectin Polysaccharide from Walnut Heartwood and Evaluation of Its Yield and Physicochemical Properties

نویسندگان [English]

  • Marjan Nouri
  • mohsen mokhtarian
Department of Food Science and Technology, Roudehen Branch, Islamic Azad University, Roudehen, Iran
چکیده [English]

Walnut is one of the most important and valuable agricultural products in the country. The aim of this study was to investigate the effects of three variables of pH values (1, 1.5 and 2), extraction temperatures (60, 70 and 80°C) and process times (60, 90 and 120 min) on yield, degree of esterification and galacturonic acid of pectin extracted from heartwood waste of walnut using response surface statistical method. Based on the results, optimum conditions for pectin extraction with the highest extraction yield (14.32%), degree of esterification (64.03%) and galacturonic acid (63.62 %) were associated with pH 1.68, process temperature of 80°C and extraction time of 120 min. Total ash, molecular weight, emulsifier, rheological and Fourier transform infrared spectroscopy assessments were carried out on pectin optimum sample. The results showed the total ash was 14.1% and MW of pectin optimum sample was 35.61 kDa. This sample, with suitable emulsifier properties, showed pseudoplastic behavior and its FTIR spectroscopy diagram was rich in galacturonic acid.

کلیدواژه‌ها [English]

  • Walnut Heart wood
  • Pectin
  • Esterification degree
  • Galacturonic acid
  • waste
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