تاثیر پیش‌فر‌آوری ویناس و ماده‌تلقیح در تولید بیوهیدروژن و ظرفیت تولید فرآورده‌های آن در خوزستان

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه مهندسی بیوسیتم، دانشکده کشاورزی، دانشگاه شهید چمران اهواز

2 گروه مهندسی بیوسیستم، دانشکده کشاورزی، دانشگاه شهید چمران اهواز

3 گروه مهندسی مکانیزاسیون کشاورزی و بیوسیستم، واحد شوشتر، دانشگاه آزاد اسلامی

4 گروه زیست‌شناسی، دانشکده علوم، دانشگاه شهید چمران اهواز

5 دانشکده مهندسی شیمی و بیوشیمی، دانشگاه صنعتی دانمارک

چکیده

ویناس، فاضلاب کارخانه­های الکل­سازی، پسماندی بسیار آلوده­کننده است. حجم زیاد ویناس و بوی نامطبوع آن، در کنار خاصیت اسیدی و بار آلودگی زیاد، آن را به یکی از مهمترین چالش­های زیست محیطی استان خوزستان بدل کرده است. کاربرد تخمیر تاریک جهت تیمار این فاضلاب، یکی از ارزان­ترین روش­های سازگار با محیط­زیست مدیریت این پسماند است. هیدروژن سوختی پاک است و در صورت تولید به روش­ بیولوژیکی، یک حامل انرژی تجدید پذیر تلقی می­گردد. در مطالعه حاضر، تولید ناپیوستة بیوهیدروژن از ویناس به روش تخمیر تاریک بررسی شده است. همچنین اثر پیش­تیمار ماده تلقیحی (لجن هضم شده) و پیش­تیمار ماده اولیه (ویناس) بر عملکرد تولید هیدروژن بررسی شد. نتایج نشان داد کاربرد کلروفرم به­عنوان پیش­تیمار ماده تلقیح با عملکرد ۶/۳ ± ۸/۴۷ نرمال میلی­لیتر/گرم جامدات فرار اولیه تاثیر بسیار معنی­داری بر افزایش عملکرد هیدروژن داشته است. همچنین کاربرد سدیم هیدروکسید تاثیر معنی داری بر هیدرولیز قند ویناس داشته و با عملکرد 1/۰ ± ۵/۹۹ نرمال میلی­لیتر/گرم جامدات فرار اولیه، بازده تولید هیدروژن را تا ۱۰۰ درصد افزود. در تمام تیمارها اسید استیک و اسید بوتیریک به­عنوان اسیدهای آلی عمده موجود در مایه نهایی تخمیر شناسایی شدند. در نهایت با توجه به نتایج آزمایشگاهی و نیز اطلاعات میدانی موجود، پتانسیل تولید بیوهیدروژن، اسید استیک و اسید بوتیریک از ویناس تولید شده در استان خوزستان در سال ۱۳۹۹ به­ترتیب به­میزان ۰۰۳/۰، ۱۵۳/۰ و ۱۳۲/۰کیلومتر مکعب محاسبه شد. نتایج نشان داد تولید بیوهیدروژن از ویناس به­روش تخمیر تاریک، با توجه به پتانسیل بالای تولید این گاز از ویناس، روشی بسیار کارآمد به­حساب می­آید.

کلیدواژه‌ها

عنوان مقاله [English]

Effect of pre-treating vinasse and inoculum on bio-hydrogen production and production capacity of its co-products in Khuzestan

نویسندگان [English]

  • Samin Baladi 1
  • Yaghoob Mansoori 2
  • Nima Nasirian 3
  • Hossein Motamedi 4
  • Irini Angelidaki 5
1 Department of Biosystem engineering Faculty of Agriculture, Shahid Chamran university of Ahvaz
2 Department of Biosystem engineering, Faculty of agriculture, Shahid Chamran university of Ahvaz
3 Department of Agricultural Mechanization and Biosystems Engineering, Shushtar Branch, Islamic Azad University
4 Department of Biology, Faculty of Sciences, Shahid Chamran University of Ahvaz
5 Department of Chemical and Biochemical Engineering, Technical university of Denmark
چکیده [English]

Abstract: Vinasse, the effluent of alcohol distilleries, is a highly polluting waste. The high volume of vinasse and its unpleasant odor, along with its acidic properties and high pollution load, has made it one of the most important environmental challenges in Khuzestan. The use of dark fermentation to treat this wastewater is one of the cheapest and most environmentally friendly methods. Hydrogen is a clean fuel and considered as a carrier of renewable energy, if produced biologically. In the present study, the batch production of bio-hydrogen from vinasse by dark fermentation was studied. The effect of inoculum pretreatment (anaerobic digested sludge) and substrate pretreatment (vinasse) on hydrogen production yield were also investigated. The results showed that the use of chloroform as an inoculum pretreatment had increased hydrogen yield very significantly which lead to a yield of 47.8 ± 3.6 Nml/g of initial volatile solids (VS). Also, sodium hydroxide application had a significant effect on vinasse sugars hydrolysis, and the hydrogen production efficiency was increased up to 100 percent by 99.5 ± 0.1 Nml/ g of initial VS. In all treatments, acetic acid and butyric acid were identified as the dominant organic acids in the final fermentation broth. Finally, according to laboratory results and available field information, the production potential of bio-hydrogen, acetic and butyric acid from. vinasse produced in Khouzestan in 2021 was calculated to be 0.003, 0.153 and 0.132 km3, respectively. The results showed that the production of bio-hydrogen from vinasse by dark fermentation is a very efficient method due to the high potential of this gas production.

کلیدواژه‌ها [English]

  • Vinasse
  • Dark fermentation
  • Pre-treatment
  • Anaerobic sludge
  • Bio-hydrogen

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مهندسی بیوسیستم ایران
دوره 53، شماره 1
اردیبهشت 1401
صفحه 1-23

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  • تاریخ دریافت: 20 خرداد 1400
  • تاریخ بازنگری: 20 فروردین 1401
  • تاریخ پذیرش: 21 فروردین 1401

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